Basic blue 9
Synonyms
CI 52015;
Methylene blue;
Methylthioninium chloride;
Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride (1:1);
Category
Cationic surfactant
> Thiazinonium Salt
Chemical Numbering System
CASRN: 61-73-4
EINECS: 200-515-2
Molecular Formula & Molecular Weight
Molecular Formula: C16H18ClN3S; C16H18N3S.Cl
Molecular Weight: 319.85
Structure
Properties
Appearance (1), solid.
Solubility soluble in water.
Physical - chemical properties
Water solubility, g/L |
43.6 (25 °C)[1] |
Stability stable.
Risk Solid form: flammable material; irritation, irritation to skin, eye, respiratory system. Harmful products of combustion are CO, CO2 and so on. Contact with strong oxidants, can cause to burn.
GHS (Rev.10) label:
Animal Toxicity
Table 1 Animal toxicity values
cat intravenous LDLo |
41mg/kg[2] |
dog intravenous LDLo |
50mg/kg[2] |
dog oral LDLo |
500mg/kg[2] |
guinea pig subcutaneous LDLo |
300mg/kg[2] |
monkey intravenous LDLo |
10mg/kg[2] |
mouse intraperitoneal LD50 |
150mg/kg[2] |
mouse intravenous LD50 |
77mg/kg[2] |
mouse oral LD50 |
3500mg/kg[2] |
rabbit oral LDLo |
1000mg/kg[2] |
rat intraperitoneal LD50 |
180mg/kg[2] |
rat intravenous LD50 |
1250mg/kg[2] |
rat oral LD50 |
1180mg/kg[2] |
rat subcutaneous LD50 |
190mg/kg[2] |
Human Toxicity
Table 2 Human toxicity values
man subcutaneous TDLo |
0.028mL/kg[2] |
Ecology may be hazardous to environment. Water body should be given special attention.
Biodegradability biodegradable.
Characteristics excellent dyeing, masking, dispersing, antistatic abilities. Thiazine dyes.
Synthesis
Quality Standards & Test Methods
1, Physical and chemical indexes
Item |
Physical and chemical indexes |
Solid |
|
Actives, %, ≥ |
90.0 |
Test Methods
(1), Actives
GB/T 13173 Surface active agents -- Detergents -- Testing methods
2, Hazardous substances indexes
Item |
Hazardous substances indexes |
Solid |
|
Heavy metals (as Pb), mg/kg, ≤ |
20 |
As, mg/kg, ≤ |
3 |
Test Methods
(1), Heavy metals (as Pb)
Safety and Technical Standards for Cosmetics (2015 Edition)
GB/T 30799 The test method of food detergents -- Determination of heavy metals
(2), As
Safety and Technical Standards for Cosmetics (2015 Edition)
GB/T 30797 The test method of food detergents -- Determination of total arsenic
3, Product standards
(1), Chinese standards
(1a), Chinese Pharmacopoeia (2020 Edition) Methylthioninium Chloride
4, Further explanation
(a), On physical and chemical indexes: firstly, shall be indicated carbon atom distribution; secondly, shall be indicated average molecular weight.
Major Uses
1, Typical applications
Use as dye.
2, Medicine
Use as pharmaceutical ingredient (antidote). (This section is for reference only, should be implemented according to local Pharmacopoeia.)
3, Reagent
Use as indicator.
Manufacturers
References
[1]: HSDB. Methylene blue, 61-73-4
[2]: ChemIDplus. Methylene blue, 61-73-4